One of the future goals for this technology is to design base polymer dyes and polymer tandem dyes that can be easily multiplexed to make multicolor flow cytometry easier. These tandem dyes are very bright compared to conventional tandem dyes. The molecules are chemically linked together to form a tandem dye that is excited at the excitation wavelength of the donor and emits at the emission wavelength of the acceptor. Polymer tandem dyes are composed of two dye molecules-the base polymer (donor) dye and a second conventional fluorescent (acceptor) dye. They are very effective at collecting excitation light (like molecular antennae) and efficiently converting it to emitted light at a longer wavelength. The base polymer dyes are the simplest embodiment of the technology, are very photostable and are bright compared to conventional fluorescent dyes. They can dramatically increase detection signals (up to 100 times) for sensitivity.īase Polymer Dyes and Polymer Tandem DyesīD Horizon Brilliant™ Dyes come in two basic formats-base polymer dyes and polymer tandem dyes. These superior polymer reporters can then be attached to nucleic acid probes and antibodies for use in a variety of assays and applications. BD Horizon Brilliant™ Polymer Dyes are based on these patented chemistries, which allow the creation of very photostable polymer dyes that are effective at collecting excitation light and efficiently converting it to emitted light at a longer wavelength. In 2000, the Nobel Prize in Chemistry was awarded for the discovery of a unique class of conducting polymer materials that are revolutionizing the electronic and display industries. The BD Horizon Brilliant™ Dyes are based on a winning technology This means that they are effective for identifying cell populations with a broader range of receptor density than previously possible. The characteristics of the BD Horizon Brilliant™ Polymer Dyes enable them to achieve, in some cases, much brighter fluorescence signals than traditional organic fluorescent dyes or even phycobiliproteins such as PE or APC. These unique dyes can be brighter than conventional dyes, with equivalent background-a breakthrough in the field.įor cells that have few receptors on the surface, bright reagents are essential in resolving these dim cells from others in a sample. BD OptiBuild™ Reagents provide flexibility to evaluate and add new fluorochromes and simplify your panel design.īD Horizon Brilliant™ Dyes were developed from pioneering polymer dye technology acquired from Sirigen Ltd. To help you choose the right fluorochromes for your experiments, we have developed a fluorochrome atlas that allows you to view and compare dyes with attributes such as excitation/emission spectra, filter recommendations and relative resolution information.īD OptiBuild™ On-Demand Reagents make it easy for you to gain access to the antibodies you need on an ever-growing catalog of dye combinations. We offer a wide variety of fluorochromes across every laser line for multicolor flow cytometry. To fully leverage the power of multicolor flow cytometry, it is critical to understand which fluorochrome to use, compare the spectral properties of different fluorochromes and determine the right instrument based on the number of parameters needed to be analyzed. Lasers provide information on the size and complexity of cells and excite fluorochromes conjugated to antigen-specific antibodies that enable the identification and understanding of the function of cells.
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We offer a comprehensive line of cell analyzers and cell sorters to support you through your research continuum. As pioneers in flow cytometry, we have been at the forefront of flow cytometry instrumentation development.